Device

Part:BBa_K1789021

Designed by: Chushu Zhu   Group: iGEM15_NUDT_CHINA   (2015-09-14)
Revision as of 09:56, 18 September 2015 by Zhuchushu13 (Talk | contribs)

IAA prducer

This is a device which contains the sequence of the recombination of TALE1 and IAAM and the recombination of TALE2 and IAAH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2724
    Illegal BamHI site found at 2311
    Illegal BamHI site found at 3669
    Illegal BamHI site found at 6072
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2431
    Illegal NgoMIV site found at 7088
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1304
    Illegal BsaI.rc site found at 5166
    Illegal BsaI.rc site found at 5370

Experimental Validation

[edit]
Categories
//awards/part_collection/2015
Parameters
chassisE. coli BL21(DE3)
control
device_typepathway
input_stryptophan
originP. savastanoi
outputindole-3-acetic acid
resistancechloramphenicol