Composite

Part:BBa_K1813027

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-08-05)
Revision as of 08:05, 18 September 2015 by Rc (Talk | contribs)

CPY6CM1 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP6CM1vQ (BBa_K1813003) under the regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 1737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2327
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6CM1vQ without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.

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Categories
Parameters
None