Composite

Part:BBa_K1813018

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)
Revision as of 06:25, 18 September 2015 by Rc (Talk | contribs)

AgambNADPH Expression Cassette

A construct used to achieve the overexpression of the A. gambiae NADPH p450 reductase (BBa_K1813010) in a bacterial host.

Structure: ptac:: AgambNADPH:: terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
    Illegal AgeI site found at 1540
    Illegal AgeI site found at 1696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2056


Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYPCM1vQ by any bacterium harboring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813028.

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Categories
Parameters
None