Coding

Part:BBa_K1640008:Design

Designed by: Meghan Cook   Group: iGEM15_Macquarie_Australia   (2015-09-10)
Revision as of 04:02, 15 September 2015 by Cherin.nazha (Talk | contribs)

psbMZH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Modularisation: This part is to be combined with psbWK, containing psbW and psbK, to form psbMZHWK, one of five operons for the biosynthesis of a minimal Photosystem II.

Overview: When induced, Escherichia coli will express the psbM, psbZ, and psbH subunits of photosystem II.

Detection Methods: PsbMZH has been detected in E. coli by gel electrophoresis, and confirmed by DNA sequencing.


Source

Chlamydomonas reinhardtii

References

Bentley, F. K., Luo, H., Dilbeck, P., Burnap, R. L., & Eaton-Rye, J. J. (2008). Effects of Inactivating psbM and psbT on Photodamage and Assembly of Photosystem II in Synechocystis sp. PCC 6803†. Biochemistry, 47(44), 11637-11646.

Ferreira, K. N., Iverson, T. M., Maghlaoui, K., Barber, J., & Iwata, S. (2004). Architecture of the Photosynthetic Oxygen-Evolving Center. Science, 303(5665), 1831-1838. doi:10.1126/science.1093087

Komenda, J., Lupínková, L., & Kopecký, J. (2002). Absence of the psbH gene product destabilizes photosystem II complex and bicarbonate binding on its acceptor side in Synechocystis PCC 6803. European Journal of Biochemistry, 269(2), 610-619. doi:10.1046/j.0014-2956.2001.02693.x

Mayes, S. R., Dubbs, J. M., Vass, I., Hideg, E., Nagy, L., & Barber, J. (1993). Further characterization of the psbH locus of Synechocystis sp. PCC 6803: inactivation of psbH impairs QA to QB electron transport in photosystem 2. Biochemistry, 32(6), 1454-1465.

Minagawa, J., & Takahashi, Y. (2004). Structure, function and assembly of Photosystem II and its light-harvesting proteins. Photosynthesis Research, 82(3), 241-263. doi:10.1007/s11120-004-2079-2

Summer, E. J., Schmid, V. H., Bruns, B. U., & Schmidt, G. W. (1997). Requirement for the H phosphoprotein in photosystem II of Chlamydomonas reinhardtii. Plant physiology, 113(4), 1359-1368.

Swiatek, M., Kuras, R., Sokolenko, A., Higgs, D., Olive, J., Cinque, G., . . . Bassi, R. (2001). The chloroplast gene ycf9 encodes a photosystem II (PSII) core subunit, PsbZ, that participates in PSII supramolecular architecture. The Plant Cell, 13(6), 1347-1368.

Umate, P., Schwenkert, S., Karbat, I., Dal Bosco, C., Mlcòchová, L., Volz, S., . . . Meurer, J. (2007). Deletion of PsbM in tobacco alters the QB site properties and the electron flow within photosystem II. Journal of Biological Chemistry, 282(13), 9758-9767.

Vener, A. V., Harms, A., Sussman, M. R., & Vierstra, R. D. (2001). Mass spectrometric resolution of reversible protein phosphorylation in photosynthetic membranes ofArabidopsis thaliana. Journal of Biological Chemistry, 276(10), 6959-6966.