Composite

Part:BBa_K1813012

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)
Revision as of 06:33, 18 September 2015 by Rc (Talk | contribs)

CYP6G1 Expression Cassette A construct used to achieve the overexpression of CYP6G1(BBa_K181304)
Structure: ptac:: CYP6G1:: terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1030


Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP6G1 by a bacterium harbouring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813025 and BBa_K1813033.

[edit]
Categories
Parameters
None