Coding
Part:BBa_K1789000
Designed by: Xinyuan Qiu Group: iGEM15_NUDT_CHINA (2015-09-12)
Revision as of 03:04, 18 September 2015 by Qiuxinyuan12 (Talk | contribs)
IaaM
This part is the coding sequence of tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.
Usage and Biology
Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from Pseudomonas savastanoi. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 402
Illegal BamHI site found at 1347 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 109
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |