Composite

Part:BBa_K1795002:Design

Designed by: Andrew D Halleran, Joseph L Maniaci   Group: iGEM15_William_and_Mary   (2015-09-17)
Revision as of 00:49, 18 September 2015 by Jlmaniaci (Talk | contribs)

R0010 gRNA under R0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function. Thanks to team iGEM13_SJTU-BioX-Shanghai for original, non-codon optimized sequence.


References