Composite
cI-Cro+

Part:BBa_K1846005

Designed by: Luba Prout, Vitor Pinheiro   Group: iGEM15_Birkbeck   (2015-09-17)
Revision as of 11:49, 17 September 2015 by Registry (Talk | contribs)

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cI-Cro bacteriophage lambda lytic cycle regulatory circuit

The cI-Cro construct contains a circuit for the production of the cI repressor protein (also known as the Lambda Repressor), which is responsible for keeping bacteriophage lambda in the lysogenic cycle through the cooperative binding of two repressor dimers to the DNA, repressing the Cro gene. The circuit uses a T7 TE promoter. This BioBrick also contains a circuit for the production of Cro governed by a T7 promoter, which turns off gene transcription during the lytic cycle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None