Protein_Domain

Part:BBa_K1781005

Designed by: Marvin Prein   Group: iGEM15_TU_Dresden   (2015-09-09)
Revision as of 15:09, 9 September 2015 by Registry (Talk | contribs)

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LZ-T25 - Leucine Zipper bound to T25 fragment of BACTH

Leucine zippers are one of the most common protein-binding motifs. The gene codes for an alpha helix that contains a high amount of leucine residues. This alpha helix can interact with another leucine zipper and dimerize. This interaction has been thoroughly characterized and confirmed as a good control. The leucine zipper here is bound to the T25 subunit of the BACTH system and can be used as a control for the T18 and T25 interactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 43


[edit]
Categories
Parameters
None