Coding

Part:BBa_K1781004

Designed by: Marvin Prein   Group: iGEM15_TU_Dresden   (2015-09-09)
Revision as of 14:24, 9 September 2015 by Registry (Talk | contribs)

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T18 - one of two fragments of the T25/T18 BACTH system

The T18 sequence fragment is one part of the T25/T18 BACTH system. This system is used to detect protein-protein interactions. Each one of the fragments are bound to a target protein, and when these proteins interact the T25 and T18 subunits are able to connect and restore the ability to produce cyclic AMP. This then induces the expression of lac-Z and thus can be detected on agar plates containing X-gal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 19
    Illegal AgeI site found at 574
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None