Coding
Part:BBa_K1692004
Designed by: Daniel Kunin Group: iGEM15_Stanford-Brown (2015-08-03)
Revision as of 22:15, 17 September 2015 by Daniel.kunin (Talk | contribs)
codon optimized PAL with T7 promoter and Flag Tag
Introduction
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.
Background
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1315
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1533
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/part_collection/2015
//chassis/prokaryote/ecoli
//collections/probiotics/production
//chassis/prokaryote/ecoli
//collections/probiotics/production
None |