Composite

Part:BBa_K1758314

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-09-08)
Revision as of 21:59, 17 September 2015 by Luzia (Talk | contribs)

copper activator under control constitutive promoter and strong RBS Chromium induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection

Usage and Biology

This part is essential for our in vitro characterization of our chromium sensor. The edition of T7 promoter to BBa_K1758313 enables us to characterize tihs sensor in our CFPS. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/metal
Parameters
None