Composite

Part:BBa_K1766002

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-09-06)
Revision as of 20:12, 17 September 2015 by Linneaos (Talk | contribs)

MicF regulated GFP

This part consists of the micF target region of the E. coli ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF.

[[File:{Fusion protein micf gfp.jpg}|{{200}x{200}px}]]

This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861

References [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12


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