Composite

Part:BBa_K1766002

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-09-06)
Revision as of 19:35, 17 September 2015 by Linneaos (Talk | contribs)

MicF regulated GFP

This part consists of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001"> micF target region</a> of the quorum synthase RhlI under control of an OmpR dependent promoter. The promoter is activated by phosphorylated OmpR protein. RhlI produces N-butyryl-homoserine lactone (BHL), a quorum sensing molecule which enables cell to cell signaling.

This part was created to convert the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015, into an extracellular signal that could be detected by a read-out strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861


[edit]
Categories
Parameters
None