Composite

Part:BBa_K1766007

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-09-10)
Revision as of 19:09, 17 September 2015 by Utsakarmakar (Talk | contribs)

OmpR Regulated RhlI/LuxI

This part is a combination of OmpR regulated RhlI (produces a quorum sensing molecule, BHL), micF regulated LuxI (produces a quorum sensing molecule, OHHL) and micF RNA. The part was designed to achieve a highly sensitive on-off switch mechanism in the OmpR dependent regulatory system.

The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce BHL in high osmolarity condition when phosphorylated OmpR expresses RhlI. In low osmolarity condition, it is supposed to produce OHHL as the micF binds to its target site, leading to LuxI expression.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1266
    Illegal NheI site found at 1289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1150
    Illegal BglII site found at 2098
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None