Reporter

Part:BBa_K1660005

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)
Revision as of 05:53, 8 September 2015 by Registry (Talk | contribs)

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RFP controlled by the PompR promoter

PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 690
    Illegal AgeI site found at 802
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/regulatory/uncategorized
//direction/forward
//function/coliroid
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
chassisE. coli TOP10
n/amRFP protein generator TERM+ RBS+(B0032)
resistanceChloramphenicol