![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K1660005
Designed by: Dai Yuanyi Group: iGEM15_BNU-CHINA (2015-09-08)
RFP controlled by the PompR promoter
PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 690
Illegal AgeI site found at 802 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/ecoli
//classic/regulatory/uncategorized
//direction/forward
//function/coliroid
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//classic/regulatory/uncategorized
//direction/forward
//function/coliroid
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
chassis | E. coli TOP10 |
n/a | mRFP protein generator TERM+ RBS+(B0032) |
resistance | Chloramphenicol |