Device
Part:BBa_K1587006
Designed by: Alexandre Le Scornet Group: iGEM15_Toulouse (2015-09-14)
pompC and lacI box regulated cI expression
Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly the final construct did not follow a circadian rhythme.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
chassis | E. coli |