Composite

Part:BBa_K1583205

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)
Revision as of 10:14, 4 September 2015 by Registry (Talk | contribs)

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High constitutive promoter + CsgB & CsgC

CsgB functions as the anchor on the cell wall for curli nanowires. It triggers the aggregation of CsgA and leads to the formation of amyloid nanowires. CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. Constructs with different promoter strenghts were designed to investigate the impact on nanowire self-assembly. Medium expression level.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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