Composite

Part:BBa_K1660009

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)
Revision as of 07:08, 8 September 2015 by Registry (Talk | contribs)

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J23100-B0034-rMpL

encodes a nematotoxic lectin from the parasol mushroom(Macrolepiota procera) under the control of a constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 113
    Illegal AgeI site found at 209
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
Parameters
chassisE. coli BL21(DE3)
device_typeβ-trefiol lectin
functionvirulent to nematode
originMacrolepiota procera
resistanceChloramphenicol
targetterminal N-acetyllactosamine and β-galactosides