Part:BBa_K1824000
Constitutive Promoter J23119 + RNA Thermometer A1
This is a composite regulatory part that consisting of constitutive promoter BBa_J23119 and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.
Figure 1: Possible secondary structure of RNAT A1.
Characterization
Figure 2: The fluorescence of J23119+A1 with different dilution ratio at 30℃ and 37℃.
As Figure 2 shows, the fluorescence of the sample at 37℃ is 12 times higher than the fluorescence of the sample at 30℃, which means the amount of protein express at 37℃ is 12 times bigger than at 30℃.
References
1. Nocker, A. et al. mRNA-based thermosensor controls expression of rhizobial heat shock genes. Nucleic Acids Res. 29, 4800–4807 (2001).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |