Device
Part:BBa_K1742013
Designed by: Ruben Escriba Piera Group: iGEM15_Valencia_UPV (2015-09-10)
35S:PhiC31:T35S - 35S:ReporterPhiC31::GFP:T35S
Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1449
Illegal NheI site found at 1619
Illegal NheI site found at 2661 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1107
Illegal BamHI site found at 1844
Illegal BamHI site found at 1874
Illegal BamHI site found at 1960
Illegal BamHI site found at 1970
Illegal XhoI site found at 1801 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1041
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2331
Illegal SapI.rc site found at 2656
[edit]
Categories
Parameters
//chassis/eukaryote/nbenthamiana
None |