Device

Part:BBa_K1742009

Designed by: Pilar Baldominos   Group: iGEM15_Valencia_UPV   (2015-09-07)
Revision as of 09:22, 7 September 2015 by Registry (Talk | contribs)

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35S:BxbI:T35S - 35S:ReporterBxBI::GFP:T35S

Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1959
    Illegal BamHI site found at 1141
    Illegal XhoI site found at 660
    Illegal XhoI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 49
    Illegal NgoMIV site found at 1460
    Illegal NgoMIV site found at 1698
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2170


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None