Coding

Part:BBa_K1742005

Designed by: Ivn Casas Rodrigo   Group: iGEM15_Valencia_UPV   (2015-09-04)
Revision as of 08:10, 4 September 2015 by Registry (Talk | contribs)

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BxBI integrase

From mycobacterium phage, it is a CDS of bxb1 integrase from serine recombinase superfamilie. It is composed by two exons and an intron from Ricinus Communis which increases integrase effciency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1556
    Illegal BamHI site found at 738
    Illegal XhoI site found at 257
    Illegal XhoI site found at 743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1057
    Illegal NgoMIV site found at 1295
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None