Coding

Part:BBa_K1678001

Designed by: Barthelemy Caron   Group: iGEM15_Paris_Bettencourt   (2015-09-16)
Revision as of 22:32, 16 September 2015 by Barth (Talk | contribs)

Pyrimidine reductase/pyrimidine deaminase cassette RibD

Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. Coding sequence has been codon optimized for Lactobacillus plantarum. Protein is expressed by the strong L.plantarum synthetic promoter BBa_K1678004. The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). The expressed protein catalyses two steps in the riboflavin pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 20
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1262


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Categories
Parameters
None