Protein_Domain

Part:BBa_K1781001

Designed by: Marvin Prein   Group: iGEM15_TU_Dresden   (2015-09-09)
Revision as of 12:42, 9 September 2015 by Registry (Talk | contribs)

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HER2 (Human Epidermal Growth Factor 2) cytoplasmic domain

HER2 is the Human Epidermal Growth Factor 2 transmembrane receptor protein. It is located on all cells and when overexpressed this oncogene is known to induce some types of cancer. In recent years, it has been used as a biomarker. In this project, protein-protein interactions were analyzed and HER2 was identified as a perfect control. Its cytoplasmic tyrosine kinase domain was known to bind to antibodies such as trastuzumab, but more recently the affibody ZHER2 was created to bind to the protein with a higher affinity. In this case, we only needed the cytoplasmic domain for our use in the analysis of the HER2-ZHER2 binding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 19
    Illegal AgeI site found at 592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 271
    Illegal BsaI.rc site found at 589
    Illegal SapI site found at 522


[edit]
Categories
Parameters
None