Coding

Part:BBa_K1668005:Design

Designed by: Xintian Xu   Group: iGEM15_ZJU-China   (2015-09-08)
Revision as of 17:56, 8 September 2015 by Registry (Talk | contribs)

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CDS tcdA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7035
    Illegal NheI site found at 7368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6469
    Illegal NgoMIV site found at 7129
    Illegal AgeI site found at 2305
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used seamless assembly.


Source

The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers metK1 and metK2

References