Regulatory
P-phlF

Part:BBa_K1725000

Designed by: Mhairi Davidson   Group: iGEM15_Glasgow   (2015-08-06)
Revision as of 17:50, 6 August 2015 by Mhairi (Talk | contribs)

PhlF repressible promoter

The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.

800px-2015-08-05_10.50.30_cropped.jpg

From left to right: I13500(B0034.E0040); R0040.I13500; K1725000.I13500; K1725020.I13500 all in pSB3K3 in DH5a cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/lactobacillus
//collections/probiotics/control
Parameters
None