Coding
Part:BBa_K1668006:Design
Designed by: Xintian Xu Group: iGEM15_ZJU-China (2015-09-08)
CDS plu0840
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used seamless assembly.
Source
The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers metK1 and metK2