Regulatory

Part:BBa_K1597000

Designed by: Marieke Mulder   Group: iGEM15_Groningen   (2015-09-09)
Revision as of 10:30, 15 September 2015 by Marieke.groningen (Talk | contribs)

Salt inducible promoter for B. subtilis

Bacillus subtilis is capable of coping with fluctuating salt concentrations. One of the genes involved is proH, this is an 1-pyrroline-5-caboxylate reductase. A study has shown that this gene is under control of the proH-promoter. The proH promoter can be activated by a range of salt concentrations, respectively ranging from 0,1M salt up to 1 M. We have cloned this promoter out of the genome of Bacillus subtilis and combined it with tasA (a gene used in forming the amyloid-like fibers of the biofilm matrix) and GFP. The latter was used to validate the promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None