Regulatory
Part:BBa_K1597000
Designed by: Marieke Mulder Group: iGEM15_Groningen (2015-09-09)
Revision as of 10:30, 15 September 2015 by Marieke.groningen (Talk | contribs)
Salt inducible promoter for B. subtilis
Bacillus subtilis is capable of coping with fluctuating salt concentrations. One of the genes involved is proH, this is an 1-pyrroline-5-caboxylate reductase. A study has shown that this gene is under control of the proH-promoter. The proH promoter can be activated by a range of salt concentrations, respectively ranging from 0,1M salt up to 1 M. We have cloned this promoter out of the genome of Bacillus subtilis and combined it with tasA (a gene used in forming the amyloid-like fibers of the biofilm matrix) and GFP. The latter was used to validate the promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 48
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |