Reporter

Part:BBa_K1655001

Designed by: Annakarin Korppoo   Group: iGEM15_Aalto-Helsinki   (2015-09-14)
Revision as of 14:30, 16 September 2015 by Jrusanen (Talk | contribs) (added missing -->)

This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.

An extra nucelotide has been added prior to this GFP brick's suffix to maintain the reading frame after fusion.

Usage and Biology

This part sequence is derived from https://parts.igem.org/Part:BBa_I13504. The part has been PCRed from BBa_I13504, and the reverse primer has added an extra guanine nucleotide prior to the birck's suffix. This will allow for easy aminoterminal GFP fusion with the biobrick enzyme assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None