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Part:BBa_K1679008:Design

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-08)
Revision as of 09:51, 16 September 2015 by Yefeng (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1679008 short</partinfo> <partinfo>BBa_K1679008 SequenceAndFeatures</partinfo> ===Design Notes=== We use a special RBS to make the device temperature-...")

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promoter+RNA thermometer+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 656
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a special RBS to make the device temperature-sensitive.When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded.Thus the ribosome can't translated RFP.However,when the temperature rises above 37°C,the secondary structure of the RBS after transform would be unfolded and produce RFP.

Source

BBa_J23101

BBa_K115001

BBa_E1010

References