Regulatory

Part:BBa_K1847009

Designed by: Anna Fomitcheva, Lisa Baumgartner, Charlotte Ramon   Group: iGEM15_ETH_Zurich   (2015-08-26)
Revision as of 12:27, 14 September 2015 by Milhene (Talk | contribs)

lldRO1-J23118-lldRO2

Collection of promoters regulated by LldR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

J23118-B0034-lldR J23118-B0034-lldP-lldR J23114-B0032-lldP-lldR
Coefficient values K=977.5 (812.6, 1142) K=2361 (1051, 3671) K=459.8 (121.5, 798)
al = 1.1e+04 (fixed at bound) al = 516.7 (-1522, 2555) al = 1.775e+04 (1.619e+04, 1.93e+04)
mu = 1.717e+04 (1.634e+04, 1.8e+04) mu = 1.256e+04 (9078, 1.603e+04) mu = 1.711e+04 (1.366e+04, 2.057e+04)
n1 = 1.084 (0.8159, 1.352) n1 = 0.7986 (0.3991, 1.198) n1 = 0.7485 (0.3491, 1.148)
Goodness to fit sse: 5.5485e+08 sse: 3.3911e+08 sse: 9.3519e+09
rsquare: 0.9967 rsquare: 0.9894 rsquare: 0.9835
fe: 8 dfe: 7 dfe: 7
adjrsquare: 0.9959 adjrsquare: 0.9848 adjrsquare: 0.9764
rmse: 8.3280e+03 rmse: 6.9602e+03 rmse: 3.6551e+04
  • Dose-response curve for our promoter with J23118-B0034-lldR
  • Dose-response curve for our promoter with J23114-B0032-lldP-lldR
  • Dose-response curve for our promoter with J23118-B0032-lldP-lldR


[edit]
Categories
//awards/part_collection/2015
Parameters
None