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T7 express

Part:BBa_K1639012:Design

Designed by: Nurgeldi Bazarov   Group: iGEM15_ATOMS-Turkiye   (2015-09-15)
Revision as of 19:15, 15 September 2015 by Nurish (Talk | contribs) (→‎Design Notes)


T7 expression system for pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 58
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 72


Design Notes

We inserted BamH1 and Sal1 cut sites between T7 promoter and T7 terminator. This part also contain T7 bacteriophage RBS. Any other team can use this part to modify vectors like pSB1C3 to add T7 expression feature. Just clone it with enzymes at prefix and suffix.

Source

It is combination of T7 promoter-terminator with sequences containing restriction enzyme cut sites.

References