Translational_Unit

Part:BBa_K1688002

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)
Revision as of 08:23, 15 September 2015 by Registry (Talk | contribs)

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RhlA Gene

The enzymes responsible for producing the mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The mono- rhamnolipids consist of a hydrophobic tail and a hydrophilic head. The RhlA gene codes for the fatty acid tail and it has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from pseudomona aeruginosa. The biobrick from team Nankai BBa_K1331001 consists of gene RhlA whereas the biobrick BBa_K1688002 consist of a ribosomal binding site and a gene coding RhlA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
    Illegal BamHI site found at 647
    Illegal XhoI site found at 823
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312
    Illegal BsaI.rc site found at 496


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Categories
Parameters
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