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Coding

Part:BBa_K1761001:Design

Designed by: Laura van Smeden   Group: iGEM15_TU_Eindhoven   (2015-09-03)
Revision as of 10:55, 3 September 2015 by Registry (Talk | contribs)

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Outer Membrane Protein X (OmpX) with BamHI-linker and mNeonGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 574
    Illegal BamHI site found at 619
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is redesigned considering codon optimalization. Also restriction sites for biobricking and classical cloning are deleted. From the BamHI linker, the coding sequecne for GGSGGS is redesigned causing that only two BamHI restriction sites remain.


Source

OmpX is a outer membrane protein naturally occuring in wildtype E.coli. The BamHI linker is inspired by the article "Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Liners" by Toon H. Evers et all from 29 August 2006. mNeonGreen naturally occurs in the organism Branchiostoma Lanceolatum.

References