Regulatory

Part:BBa_K1668004:Design

Designed by: Xintian Xu   Group: iGEM15_ZJU-China   (2015-09-08)
Revision as of 17:48, 8 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ermEp (a strong constitutive promoter in S. avermitilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use seamless assembly.


Source

The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers metK1 and metK2（

References