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Part:BBa_K1766009

Designed by: Pontus Höjer   Group: iGEM15_Stockholm   (2015-09-13)
Revision as of 14:56, 13 September 2015 by Registry (Talk | contribs)

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BAR construct 1

BAR construct 1 was created in the hope of making a receptor able to signal binding to an antigen. By making a chimera between a histidine kinase (EnvZ) and a affibody molecule (Z:HER2:342) we would have a receptor capable of detecting the HER2 (human epidermal growth factor receptor) protein biomarker, commonly used for detecting breast cancer. The construct would use the EnvZ-OmpR two-component regulatory system to signal binding and activation of the histidine kinase. However successful signaling has not been documented.

The construct has been studied for expression, pressence in membrane and affinity towards HER2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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