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Part:BBa_K1766008
EnvZ_V5 osmoregulatory histidine kinase from E.coli.
EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 316
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 316
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 316
Illegal BglII site found at 236 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 316
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 316
- 1000COMPATIBLE WITH RFC[1000]
Experiments
Experssion
Osmolarity tests
References
[1] Russo, F. D. (1992) Ph.D. thesis. Princeton University, Princeton, N.J.
[2] Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A. (2005) Protein Identification
and Analysis Tools on the ExPASy Server; (In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press
[3] Nørholm M. H. H., von Heijne G., and Draheim R. R. (2014) Forcing the Issue: Aromatic Tuning Facilitates Stimulus-Independent Modulation of a Two-Component Signaling Circuit. ACS Synth. Biol. 2015, 4, 474−481
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