Coding

Part:BBa_K1766008

Designed by: Pontus Höjer   Group: iGEM15_Stockholm   (2015-09-13)
Revision as of 13:21, 14 September 2015 by Pontushojer (Talk | contribs)

EnvZ_V5 osmoregulatory histidine kinase from E.coli.

EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiments

Experssion

Osmolarity tests


References

[1] Russo, F. D. (1992) Ph.D. thesis. Princeton University, Princeton, N.J.
[2] Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A. (2005) Protein Identification and Analysis Tools on the ExPASy Server; (In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press
[3] Nørholm M. H. H., von Heijne G., and Draheim R. R. (2014) Forcing the Issue: Aromatic Tuning Facilitates Stimulus-Independent Modulation of a Two-Component Signaling Circuit. ACS Synth. Biol. 2015, 4, 474−481

[edit]
Categories
Parameters
None