Coding
Part:BBa_K1597003
Designed by: Marieke Mulder Group: iGEM15_Groningen (2015-09-14)
bslA, forms hydrophobic surface layer on biofilm
To make our biofilm more robust and hydrophobic we we have focussed on bslA (K-nummer). bslA forms a hydrophobic surface layer on the biofilm of Bacillus subtilis. This layer is essential for the water repelling properties of the biofilm of the Bacillus subtilis. The BslA protein itself is a cell wall protein with amphiphilic properties which self-assembles into polymers at the air-water interface in vitro. For the expression of bslA, expression of epsABCDEFGHIJKLMNO and tapA-sipW-tasA operons are required. Without bslA the microstructure of the biofilm is smoothened and the repellency against water and organic solvents is reduced.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |