Coding

Part:BBa_K1766008

Designed by: Pontus Höjer   Group: iGEM15_Stockholm   (2015-09-13)
Revision as of 13:53, 13 September 2015 by Registry (Talk | contribs)

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EnvZ_V5 osmoregulatory histidine kinase from E.coli.

EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes a C-terminal V5-tag through a GGSSAAG linker. The part was used in a pRD400 backbone behind a lac-inducible promoter to study signaling as well as expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None