Device

Part:BBa_K1668008

Designed by: Yang Su   Group: iGEM15_ZJU-China   (2015-09-08)
Revision as of 13:11, 14 September 2015 by Runnersy (Talk | contribs)

tcdA1-device

the part tcdA1 device is composed of arabinose inducible promoter pBad BBa_I0500, toxin protein tcdA1 coding sequnceBBa_K1668008 and composite part mCherry BBa_K1668011.

We use the device to tandem express toxic protein tcdA1 and mCherry. Toxic protein tcdA1 is a macro channel forming toxin used for termite control in our project and mCherry is a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 8257
    Illegal NheI site found at 8590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1651
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7691
    Illegal NgoMIV site found at 8351
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None