Coding

Part:BBa_K1595030:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)
Revision as of 02:13, 14 September 2015 by Registry (Talk | contribs)

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EDA linked to cel5a via GSG linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 641
    Illegal NgoMIV site found at 1038
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1755


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

cel5a is a stable, detectable protein that can be used to determine expression of our plasmid.


Source

Synthesize by DNA 2.0

References