Coding

Part:BBa_K1819001:Design

Designed by: Lais Ribovski   Group: iGEM15_Brasil-USP   (2015-09-13)
Revision as of 03:05, 14 September 2015 by Laisribovski (Talk | contribs) (→‎Source)

Latex Clearing Protein (Lcp) - rubber degradation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 889
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438


Design Notes

Because Lcp is secreted by Streptomyces sp, it contains a 5’-signal peptide that was identified using signal peptide predictor server.[1] We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites

Source

K1819001 source Not understood 

Lcp from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1).

References

1. Bagos PG, Nikolaou EP, Liakopoulos TD, Tsirigos KD. Combined prediction of Tat and Sec signal peptides with Hidden Markov Models. Bioinformatics. 2010; 6(22):2811-2817.