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Zasp

Part:BBa_K1620001:Design

Designed by: Celio Dias Santos Jr   Group: iGEM15_UFSCar-Brasil   (2015-09-03)
Revision as of 19:31, 3 September 2015 by Registry (Talk | contribs)

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Promoter element Zasp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have identified two sequences of this promoter (one in sense strand and another in the antisense strand). In order to turn this sequence more feasible we used a hybrid final sequence. The final sequence was believed to promote for both sites.


Source

The promoter element was designed through bioinformatic analysis of 500bp frame upstream genes of operon ZnuABC from E. coli K12 MG16555 genome. Then, it was synthesized by IDT through G-block and further assembled by 3A assembly method.

References