Coding

Part:BBa_K1699004:Design

Designed by: Emil Ruvinov   Group: iGEM15_BGU_Israel   (2015-08-09)
Revision as of 19:57, 5 September 2015 by Shalevgo (Talk | contribs) (→‎Source)


gRNA for SaCas9 targeting human ubiquitin B gene under U6 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSBC13 using EcoRI and PstI restriction sites.
F. tagtcatggcggccgcgtcgacG
R. actgacatgcggccgcttaattaaCTGC

Source

gRNA for UBB by IDT de-novo syntesis.

References

1. In vivo genome editing using Staphylococcus aureus Cas9.
http://10.1038/nature14299