Coding

Part:BBa_K1699003:Design

Designed by: Emil Ruvinov   Group: iGEM15_BGU_Israel   (2015-08-09)
Revision as of 10:33, 13 September 2015 by Emilr78 (Talk | contribs) (→‎References)

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSBC13 using EcoRI and PstI restriction sites.
F. tagtcatgGAATTCGCGGCCGCTTCTAG
R. tgtatactCTGCAGCGGCCGCTAC

Source

IDT de-novo synthesis.