Translational_Unit

Part:BBa_K622000

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-01)
Revision as of 14:47, 1 October 2011 by Registry (Talk | contribs)

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rpoN: coding sigma 54 factor

This part codes sigma 54 factor of E.coli and its RBS. We use this part to make nutritional switch which is activated under poor availability of combined nitrogen. The complex of sigma 54 factor and the other transcription factor activates sigma 54 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 591
    Illegal BamHI site found at 554
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 250


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Categories
Parameters
None