Reporter

Part:BBa_K1606029

Designed by: Dorukcan Özköse   Group: iGEM15_METU_Turkey   (2015-08-27)
Revision as of 00:12, 12 September 2015 by Ilkem.kumru (Talk | contribs)

Gluten Updated m-cherry reporter gene

we modernized m-cherry (BBa_J06504) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment.

mCherry(restricted)-GFP-GFP

319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG

KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry

Kumma123metu.PNG Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 567
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


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