Generator

Part:BBa_K1773022

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-27)
Revision as of 08:03, 27 August 2015 by Registry (Talk | contribs)

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Cascade with pLux/cI right promoter and strong RBS

I-F type Cascade complex of I-F type CRISPR-Cas system. The Cascade complex is comprised of five proteins : Csy1, Csy2, Csy3 and Cas6f and an crRNA molecule. The Cascade complex distinguishes target DNA , and recruits Cas3 (BBa_K1773003) protein, which degrades the target DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2408


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Categories
Parameters
None