Composite
Part:BBa_K1604020
Designed by: Elisa Godino Group: iGEM15_UNITN-Trento (2015-08-18)
araC-pBAD + beta-carotene
This Composite Biobrick is created by standard assembly of Part BBa_K731201 and Part BBa_K274200, which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1C3 (high copy, Chloramphenicol resistance). BBa_K274200 contains the enzymes that convert colourless farnesyl pyrophosphate to orange beta-carotene. The carotene operon contains CrtE, CrtB, CrtI and CrtY each one with individual ribosome binding site. This Composite Biobrick has been put under inducible araC-pBAD promoter that is positively regulated by arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 3185 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2721
Illegal NgoMIV site found at 2851
Illegal AgeI site found at 979
Illegal AgeI site found at 1936 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
n/a | araC-pBAD + beta-carotene |